Integrated High-Throughput Centrifugal Microfluidic Chip Device for Pathogen Detection On-Site

Abstract

An integrated and high-throughput device for pathogen detection is crucial in point-of-care testing (POCT), especially for early diagnosis of infectious diseases and preventing the spread of infection. We developed an on-site testing platform that utilizes a centrifugal microfluidic chip and automated device to achieve high-throughput detection. The low-power (E. coli, our platform achieves a limit of detection (LOD) of 102 CFU/mL in 60 min. In summary, our active centrifugal microfluidic platform provides a solution for the integration of complex biological assays on turntables, with great potential in the application of point-of-care diagnosis.

Keywords

• centrifugal microfluidic chip
• nucleic acid extraction
• lamp
• paraffin valve
• hydrophobic valve
• siphon valve