Changes in Serum Blood Parameters in Farmed Rainbow Trout (<i>Oncorhynchus mykiss</i>) Fed with Diets Supplemented with Waste Derived from Supercritical Fluid Extraction of Sweet Basil (<i>Ocimum basilicum</i>)

Abstract

The antimicrobial and immunostimulant proprieties of aromatic plant extracts have been widely described, but their effects on serum blood biochemistry in fish have not. For this study, we assessed the changes in serum blood biochemical parameters in rainbow trout fed with a fish diet supplemented with a basil supercritical extract (F1-BEO). Our hypothesis was that treatment and time would be associated with changes in 10 serum blood biochemical parameters. F1-BEO was added to a commercial feed (0.5, 1, 2, 3% w/w). The fish were fed for 30 days, and the blood samples were collected at 2 time points (15 and 30 days). A two-way ANOVA showed a significant effect of treatment, time, and interaction treatment × time on creatinine, urea, total protein, albumin, magnesium, and phosphorus (p p w/w F1-BEO (p w/w indicated a positive effect of basil on liver health. Our findings suggest a potential use for basil extracts (for example., F1-BEO) in fish feed to reduce antibiotic use and improve fish welfare.

Keywords

• antimicrobials
• cholesterol
• glutamic oxaloacetic transaminase
• glutamic pyruvic transaminase
• phytotherapy