Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs

Min Zhou; Rui Wei; Yu Jiang; Jian Fu; Yuqing Liu; Bo Yang; Baorong Yu; Yun Lin; Xinru Ran; Wing-Hon Lai; Meng Chu; Yang Hu; Jiayin Yang; Hung-Fat Tse

Stem Cell Research (Jan 2021)

Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs

Min Zhou,
Rui Wei,
Yu Jiang,
Jian Fu,
Yuqing Liu,
Bo Yang,
Baorong Yu,
Yun Lin,
Xinru Ran,
Wing-Hon Lai,
Meng Chu,
Yang Hu,
Jiayin Yang,
Hung-Fat Tse

Affiliations

Min Zhou: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China
Rui Wei: Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China; Department of Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China
Yu Jiang: Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China
Jian Fu: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China
Yuqing Liu: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China
Bo Yang: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China
Baorong Yu: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China
Yun Lin: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China
Xinru Ran: Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China
Wing-Hon Lai: Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China
Meng Chu: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China
Yang Hu: Cell Inspire Therapeutics Co., Ltd., Shenzhen 518101, China
Jiayin Yang: Cell Inspire Biotechnology Co., Ltd., Shenzhen 518101, China; Cell Inspire Therapeutics Co., Ltd., Shenzhen 518101, China; Corresponding authors at: Cell Inspire Therapeutics Co., Ltd., Shenzhen 518101, China (J. Yang). Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China (H.-F. Tse).
Hung-Fat Tse: Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China; Department of Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen 518053, China; Corresponding authors at: Cell Inspire Therapeutics Co., Ltd., Shenzhen 518101, China (J. Yang). Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China (H.-F. Tse).

Journal volume & issue: Vol. 50
p. 102120

Abstract

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The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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