In-vitro GLP-1 Release Assay Using STC-1 Cells

Liu Qi; Tan Shuai; Jia Da; Burstein Ezra; Sifuentes-Dominguez Luis

doi:10.21769/BioProtoc.3717

Bio-Protocol (Aug 2020)

In-vitro GLP-1 Release Assay Using STC-1 Cells

Liu Qi,
Tan Shuai,
Jia Da,
Burstein Ezra,
Sifuentes-Dominguez Luis

Affiliations

Liu Qi: Department of Internal Medicine, Division of Digestive and Liver Diseases, University of Texas Southwestern Medical Center, Dallas, United States
Tan Shuai: Department of Internal Medicine, Division of Digestive and Liver Diseases, University of Texas Southwestern Medical Center, Dallas, United States
Jia Da: Key Laboratory of Birth Defects and Related Diseases of Women and Children, Department of Pediatrics West China Second University Hospital, State Key Laboratory of Biotherapy and Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu, China
Burstein Ezra: Department of Internal Medicine, Division of Digestive and Liver Diseases, University of Texas Southwestern Medical Center, Dallas, United States
Sifuentes-Dominguez Luis: Department of Pediatrics, Division of Pediatric Gastroenterology, Hepatology and Nutrition, University of Texas Southwestern Medical Center, Dallas, United States

DOI: https://doi.org/10.21769/BioProtoc.3717
Journal volume & issue: Vol. 10, no. 16

Abstract

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Enteroendocrine cells (EECs) are known chemosensors in the gastrointestinal (GI) epithelium. They release a diversity of gut hormones in response to various stimuli. Here, we report an in-vitro assay to measure GLP-1 release from cultured murine EEC’s under fatty acid stimulation.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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