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BioTechniques
(Sep 2019)
Caution: Plasmid DNA topology affects luciferase assay reproducibility and outcomes
Emma Tudini,
Lez J Burke,
Phillip J Whiley,
Jan Sevcik,
Amanda B Spurdle,
Melissa A Brown
Affiliations
Emma Tudini
1School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia
Lez J Burke
1School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia
Phillip J Whiley
1School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia
Jan Sevcik
1School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia
Amanda B Spurdle
2Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia
Melissa A Brown
1School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia
DOI
https://doi.org/10.2144/btn-2019-0060
Journal volume & issue
Vol. 67, no. 3
pp. 94 – 96
Abstract
Read online
No abstracts available.
Keywords
assays
cell culture
gene expression analysis
in vitro models
transfection
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