Separation of Microspores from Anthers of Lilium longiflorum (Lily) and Subsequent RNA Extraction

Ming-Che Liu; Yun-Chu Chen; Co-Shine Wang

doi:10.21769/BioProtoc.1400

Bio-Protocol (Feb 2015)

Separation of Microspores from Anthers of Lilium longiflorum (Lily) and Subsequent RNA Extraction

Ming-Che Liu,
Yun-Chu Chen,
Co-Shine Wang

Affiliations

Ming-Che Liu: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
Yun-Chu Chen: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan
Co-Shine Wang: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan

DOI: https://doi.org/10.21769/BioProtoc.1400
Journal volume & issue: Vol. 5, no. 4

Abstract

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This protocol has been designed in order to facilitate the isolation and extraction of total RNA from microspores collected from lily anther sacs. This protocol allows the extraction of high amounts of high quality RNA, as observed in agarose gels.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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