Evaluation of karyopathic effects and general safety of <i>Trichinella spiralis</i> and <i>Echinococcus multilocularis</i> extracts in mice

Abstract

The purpose of the research is assessment of karyopathic effect and general safety of Trichinella spiralis and Echinococcus multilocularis protein extracts in mice.Materials and methods. To assess the karyotic effect bone marrow cells were isolated and preparations were prepared using a metaphase method (Ford C. E., Hamerton J. L., 1956). Cytological analysis of bone marrow smears was performed to determine the white cell lineage counts. The main hematological indices of mice were determined at hematological analyzer «Abacus JuniorVet» («Diatron», Austria); differential white blood cell count was analyzed using common method. Blood biochemical indices were determined at biochemical analyzer A-15/25 (BioSystems S.A., Spain).Results and discussion. The intraperitoneal administration of T. spiralis and E. multilocularis protein extracts induced K-mitosis in mice bone marrow cell population. Systemic disorders associated with that cytogenetic effect and general reaction of organism to administration of protein extracts, in particular changes in bone marrow white cell lineage counts and peripheral blood indices were revealed. T. spiralis and E. multilocularis protein products showed some negative effects on liver and kidney function in mice.

Keywords

• <i>trichinella spiralis
• echinococcus multilocularis</i>
• karyopathic and cytogenetic effects
• mice