Diagnosis of biocenosis of the vagina of women with recurrent miscarriages

Abstract

We investigated the vaginal microbiome of 67 patients with threatened pregnancy using bacterioscopic method and qPCR of vaginal swabs, vaginal pH measurement, and FISH to evaluate desquamated epithelial cells of vagina in urine sediments. Vaginal dysbiosis of women with miscarriage was detected by evaluation of vaginal pH, vaginal swabs bacterioscopy, qPCR in 59,7%, 62,7% and 52,7% of women respectively. Gardnerella vaginalis associated vaginal dysbiosis was determined by qPCR and FISH in 14,9% of women. Enterobacteriaceae in urine sediments were highly presented in women with vaginal dysbiosis (54,8%) and pelvic and/or abdominal cavity inflammatory diseases (46,2%).

Keywords

• дисбиоз влагалища
• невынашивание беременности
• рН-метрия влагалища
• ПЦР в реальном времени
• флуоресцентная гибридизация in situ (FISH)
• гарднереллезные биопленки
• энтеробактерии