Genetically Engineered Mouse Model of Brainstem High-Grade Glioma

Fernando M. Nunez; Jessica C. Gauss; Flor M. Mendez; Santiago Haase; Pedro R. Lowenstein; Maria G. Castro

STAR Protocols (Dec 2020)

Genetically Engineered Mouse Model of Brainstem High-Grade Glioma

Fernando M. Nunez,
Jessica C. Gauss,
Flor M. Mendez,
Santiago Haase,
Pedro R. Lowenstein,
Maria G. Castro

Affiliations

Fernando M. Nunez: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding author
Jessica C. Gauss: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding author
Flor M. Mendez: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Santiago Haase: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Pedro R. Lowenstein: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
Maria G. Castro: Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA; Corresponding author

Journal volume & issue: Vol. 1, no. 3
p. 100165

Abstract

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Summary: Brainstem gliomas are aggressive tumors that are more prevalent in pediatric patients. The location of these tumors makes them inoperable, and currently there is no effective treatment. Recent genomic data revealed the unique biology of these tumors. The following protocol provides a method to incorporate these specific genetic lesions in a mouse glioma model. Using this model, the effects of these mutations in tumor progression and response to treatments can be studied within a relevant in vivo context.For complete details on the use and execution of this protocol, please refer to Mendez et al. (2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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