Investigation into Platelet Rich Plasma (PRP) Infusions Within the Intrauterine Cavity, to Support the Development of Endometrial Lining in Transfer Cycle Patients

Abstract

Background: Poor endometrial lining is classically referenced as <6mm. Patients commencing Embryo Transfer (ET) cycles can experience issues with lining development and require additional therapy supplementation with the aim of increasing lining thickness prior to ET day. PRP is an adjuvant IVF treatment, known as an infusion therapy, for use in uterine lining development support with the specific aim of increasing endometrial lining outcomes for patients prior to ET. Aim: To assess the effectiveness of PRP infusion therapy on lining development for ET cycle patients. Method: Using the TGA approved Alocuro PRP medical device system, 10mls of whole blood is collected via venepuncture, this sample is centrifuged, to produce 3 identifiable layers; Plasma, Buffy coat and Packed red blood cells. The Buffy coat is collected with 1ml of Plasma and administered via Intrauterine Infusion with an Embryo Transfer Catheter set. Patients are offered this procedure based on clinical histories of poor lining outcomes. Retrospective analysis of all patients undertaking PRP infusion, included assessment of uterine thickness, was conducted. Results: A total of 35 records were identified, of these 2 were cancelled prior to administration of PRP and 7 were removed due to incomplete records of Initial Lining measurement or Final Lining measurement. In the remaining cohort of 26 patients, 2 patients demonstrated regression in their lining, while 24 patients recorded an increase in final lining measurement, with a mean increase of 2mm or 28% over initial lining measurements. When compared with their previous cycle, PRP infusion cycles demonstrated a greater increase in lining thickness between scans, 0.82mm and 1.78mm respectively (p=0.018). Conclusion: PRP treatment appears to show potential in the treatment of poor lining development and warrants further study.