Generation of an induced pluripotent stem cell line (KEIUi008-A) from a hearing loss patient with an A1555G mutation in mitochondrial DNA

Yusuke Masano; Chika Saegusa; Mitsuru Ishikawa; Tatsuo Matsunaga; Hideyuki Okano; Masato Fujioka

Stem Cell Research (Aug 2024)

Generation of an induced pluripotent stem cell line (KEIUi008-A) from a hearing loss patient with an A1555G mutation in mitochondrial DNA

Yusuke Masano,
Chika Saegusa,
Mitsuru Ishikawa,
Tatsuo Matsunaga,
Hideyuki Okano,
Masato Fujioka

Affiliations

Yusuke Masano: Department of Molecular Genetics, Kitasato University School of Medicine, Kanagawa, Japan
Chika Saegusa: Department of Molecular Genetics, Kitasato University School of Medicine, Kanagawa, Japan; Molecular Genetics Unit, Kitasato University Graduate School of Medical Science, Kanagawa, Japan; Department of Otolaryngology, Head and Neck Surgery, Keio University School of Medicine, Tokyo, Japan
Mitsuru Ishikawa: Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Division of CNS Regeneration and Drug Discovery, International Center for Brain Science, Fujita Health University, Aichi, Japan
Tatsuo Matsunaga: Division of Hearing and Balance Research, National Institute of Sensory Organs, NHO Tokyo Medical Center, Tokyo, Japan; Medical Genetics Center, NHO Tokyo Medical Center, Tokyo, Japan
Hideyuki Okano: Department of Physiology, Keio University School of Medicine, Tokyo, Japan; Division of CNS Regeneration and Drug Discovery, International Center for Brain Science, Fujita Health University, Aichi, Japan; Keio University Regenerative Medicine Research Center, Kanagawa, Japan
Masato Fujioka: Department of Molecular Genetics, Kitasato University School of Medicine, Kanagawa, Japan; Molecular Genetics Unit, Kitasato University Graduate School of Medical Science, Kanagawa, Japan; Department of Otolaryngology, Head and Neck Surgery, Keio University School of Medicine, Tokyo, Japan; Clinical and Translational Research Center, Keio University Hospital, Tokyo, Japan; Corresponding author at: Department of Molecular Genetics, Kitasato University School of Medicine, Kanagawa, Japan.

Journal volume & issue: Vol. 78
p. 103452

Abstract

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We report the establishment of a human induced pluripotent stem cell (iPSC) line from a 54-year-old male patient with an A1555G mutation in the mitochondrial 12S ribosomal RNA gene (MTRNR1), associated with sensorineural hearing loss. The established iPSC line expressed stemness markers or undifferentiated state markers. We also demonstrated the capacity of the cells to differentiate into the three germ layers, suggesting its pluripotency and utility in the pathological study of sensorineural hearing loss and drug screening for ear disorders.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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