Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

Mary Jasmine D. Lara; Sissy E. Wamaitha; Auriana Arabpour; Jon D. Hennebold; Amander T. Clark; Enrique Sosa

Stem Cell Research (Dec 2023)

Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

Mary Jasmine D. Lara,
Sissy E. Wamaitha,
Auriana Arabpour,
Jon D. Hennebold,
Amander T. Clark,
Enrique Sosa

Affiliations

Mary Jasmine D. Lara: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA
Sissy E. Wamaitha: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; UCLA Center for Reproductive Science, Health and Education, Los Angeles, CA 90095, USA
Auriana Arabpour: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; UCLA Center for Reproductive Science, Health and Education, Los Angeles, CA 90095, USA
Jon D. Hennebold: Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon Department of Obstetrics and Gynecology, Oregon Health& Science University, Portland, OR 97006, USA
Amander T. Clark: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; UCLA Center for Reproductive Science, Health and Education, Los Angeles, CA 90095, USA
Enrique Sosa: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; UCLA Center for Reproductive Science, Health and Education, Los Angeles, CA 90095, USA; Corresponding author.

Journal volume & issue: Vol. 73
p. 103241

Abstract

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We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPSC05. The authenticity of riPSC05 was confirmed through the expression of pluripotent and self-renewal markers, in vitro-directed differentiation towards three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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