A thiol probe for measuring unfolded protein load and proteostasis in cells

Moore Z. Chen; Nagaraj S. Moily; Jessica L. Bridgford; Rebecca J. Wood; Mona Radwan; Trevor A. Smith; Zhegang Song; Ben Zhong Tang; Leann Tilley; Xiaohong Xu; Gavin E. Reid; Mahmoud A. Pouladi; Yuning Hong; Danny M. Hatters

doi:10.1038/s41467-017-00203-5

Nature Communications (Sep 2017)

A thiol probe for measuring unfolded protein load and proteostasis in cells

Moore Z. Chen,
Nagaraj S. Moily,
Jessica L. Bridgford,
Rebecca J. Wood,
Mona Radwan,
Trevor A. Smith,
Zhegang Song,
Ben Zhong Tang,
Leann Tilley,
Xiaohong Xu,
Gavin E. Reid,
Mahmoud A. Pouladi,
Yuning Hong,
Danny M. Hatters

Affiliations

Moore Z. Chen: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Nagaraj S. Moily: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Jessica L. Bridgford: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Rebecca J. Wood: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Mona Radwan: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Trevor A. Smith: School of Chemistry, The University of Melbourne
Zhegang Song: Department of Chemistry, The Hong Kong University of Science & Technology
Ben Zhong Tang: Department of Chemistry, The Hong Kong University of Science & Technology
Leann Tilley: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Xiaohong Xu: Translational Laboratory in Genetic Medicine, Agency for Science, Technology and Research
Gavin E. Reid: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Mahmoud A. Pouladi: Translational Laboratory in Genetic Medicine, Agency for Science, Technology and Research
Yuning Hong: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne
Danny M. Hatters: Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne

DOI: https://doi.org/10.1038/s41467-017-00203-5
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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Proteostasis is maintained through a number of molecular mechanisms, some of which function to protect the folded state of proteins. Here the authors demonstrate the use of TPE-MI in a fluorigenic dye assay for the quantitation of unfolded proteins that can be used to assess proteostasis on a cellular or proteome scale.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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