Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues

WU Xuan; KUANG Gang; REN Libing; GUO Wei; LIANG Jia; KANG Xiaoliang; GUO Yanli; DONG Zhiming

doi:10.3971/j.issn.1000-8578.2018.18.0586

Zhongliu Fangzhi Yanjiu (Dec 2018)

Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues

WU Xuan,
KUANG Gang,
REN Libing,
GUO Wei,
LIANG Jia,
KANG Xiaoliang,
GUO Yanli,
DONG Zhiming

Affiliations

WU Xuan: Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
KUANG Gang: Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
REN Libing: Department of Thoracic Surgery, Handan Central Hospital, Handan 056001, China
GUO Wei: Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
LIANG Jia: Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
KANG Xiaoliang: Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
GUO Yanli: Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
DONG Zhiming: Laboratory of Pathology, Hebei Cancer Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China

DOI: https://doi.org/10.3971/j.issn.1000-8578.2018.18.0586
Journal volume & issue: Vol. 45, no. 12
pp. 976 – 980

Abstract

Read online

Objective To detect the expression and methylation status of long non-coding RNA ZNF667-AS1 in esophageal squamous cell carcinoma(ESCC) tissues. Methods qPCR and MSP Methods were respectively applied to detect the expression and methylation status of ZNF667-AS1 in esophageal cancer cell lines (Kyse170, Eca109, TE1, TE13), ESCC and corresponding noncancerous tissues before or after DNA methyltransferase inhibitor 5-Aza-dC treatment. Results After treated with 5-Aza-dC, ZNF667-AS1 performed higher expression in the four esophageal cancer cell lines, and the methylation status of ZNF667-AS1 were decreased. The expression of ZNF667-AS1 in ESCC tissues was significantly lower than that in the corresponding normal tissues, and the methylation frequency of ZNF667-AS1 distal and exon1 regions CpG islands were all significantly different(P < 0.05). The methylation frequency of ZNF667-AS1 proximal promoter region CpG island in ESCC tissues was significantly higher than that in the corresponding normal tissues, and closely correlated with pathological differentiation and TNM stages; the methylation frequency in the low ZNF667-AS1 expression group was significantly higher than that in the high ZNF667-AS1 expression group. Conclusion The low expression of ZNF667-AS1 is closely related to the occurrence and development of ESCC, and its proximal promoter region methylation may be one of the mechanisms of ZNF667-AS1 silence.

Published in Zhongliu Fangzhi Yanjiu

ISSN: 1000-8578 (Print)
Publisher: Magazine House of Cancer Research on Prevention and Treatment
Country of publisher: China
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://www.zlfzyj.com

About the journal

Abstract

Keywords