Characterization of an Omega-3 Desaturase From Phytophthora parasitica and Application for Eicosapentaenoic Acid Production in Mortierella alpina

Xin Tang; Xin Tang; Haiqin Chen; Haiqin Chen; Tiantian Mei; Tiantian Mei; Chengfeng Ge; Chengfeng Ge; Zhennan Gu; Zhennan Gu; Hao Zhang; Hao Zhang; Yong Q. Chen; Yong Q. Chen; Yong Q. Chen; Yong Q. Chen; Wei Chen; Wei Chen; Wei Chen; Wei Chen

doi:10.3389/fmicb.2018.01878

Frontiers in Microbiology (Aug 2018)

Characterization of an Omega-3 Desaturase From Phytophthora parasitica and Application for Eicosapentaenoic Acid Production in Mortierella alpina

Xin Tang,
Xin Tang,
Haiqin Chen,
Haiqin Chen,
Tiantian Mei,
Tiantian Mei,
Chengfeng Ge,
Chengfeng Ge,
Zhennan Gu,
Zhennan Gu,
Hao Zhang,
Hao Zhang,
Yong Q. Chen,
Yong Q. Chen,
Yong Q. Chen,
Yong Q. Chen,
Wei Chen,
Wei Chen,
Wei Chen,
Wei Chen

Affiliations

Xin Tang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Xin Tang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Haiqin Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Haiqin Chen: School of Food Science and Technology, Jiangnan University, Wuxi, China
Tiantian Mei: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Tiantian Mei: School of Food Science and Technology, Jiangnan University, Wuxi, China
Chengfeng Ge: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Chengfeng Ge: School of Food Science and Technology, Jiangnan University, Wuxi, China
Zhennan Gu: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Zhennan Gu: School of Food Science and Technology, Jiangnan University, Wuxi, China
Hao Zhang: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Hao Zhang: School of Food Science and Technology, Jiangnan University, Wuxi, China
Yong Q. Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Yong Q. Chen: School of Food Science and Technology, Jiangnan University, Wuxi, China
Yong Q. Chen: National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China
Yong Q. Chen: Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC, United States
Wei Chen: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
Wei Chen: School of Food Science and Technology, Jiangnan University, Wuxi, China
Wei Chen: National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China
Wei Chen: Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, China

DOI: https://doi.org/10.3389/fmicb.2018.01878
Journal volume & issue: Vol. 9

Abstract

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Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) have important therapeutic and nutritional benefits in humans. In the biosynthesis pathways of these LC-PUFAs, omega-3 desaturase plays a critical role. In this study, we report a new omega-3 desaturase (PPD17) from Phytophthora parasitica. This desaturase shares high similarities with the known omega-3 desaturases and was expressed in Saccharomyces cerevisiae for the activity and substrate specificity research. The desaturase has a wide omega-6 fatty acid substrate, containing both 18C and 20C fatty acids, and exhibits a strong activity of delta-17 desaturase but a weak activity of delta-15 desaturase. The new desaturase converted the omega-6 arachidonic acid (AA, C20:4) to EPA (an omega-3 LC-PUFA, C20:5) with a substrate conversion rate of 70%. To obtain a high EPA-producing strain, we transformed PPD17 into Mortierella alpina, an AA-producing filamentous fungus. The EPA content of the total fatty acids in reconstruction strains reached 31.5% and was followed by the fermentation optimization of the EPA yield of up to 1.9 g/L. This research characterized a new omega-3 desaturase and provides a possibility of industrially producing EPA using M. alpina.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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