Comparative secretome analysis of human follicular dermal papilla cells and fibroblasts using shotgun proteomics

Abstract

The dermal papilla cells (DPCs) of hair follicles are known tosecrete paracrine factors for follicular cells. Shotgun proteomicanalysis was performed to compare the expression profiles ofthe secretomes of human DPCs and dermal fibroblasts (DFs).In this study, the proteins secreted by DPCs and matched DFswere analyzed by 1DE/LTQ FTICR MS/MS, semi-quantitativelydetermined using emPAI mole percent values and then characterizedusing protein interaction network analysis. Among the1,271 and 1,188 proteins identified in DFs and DPCs, respectively,1,529 were further analyzed using the IngenuityPathway Analysis tool. We identified 28 DPC-specific extracellularmatrix proteins including transporters (ECM1, A2M),enzymes (LOX, PON2), and peptidases (C3, C1R). The biochemically-validated DPC-specific proteins included thrombospondin1 (THBS1), an insulin-like growth factor binding protein3(IGFBP3), and, of particular interest, an integrin beta1subunit (ITGB1) as a key network core protein. Using the shotgunproteomic technique and network analysis, we selectedITGB1, IGFBP3, and THBS1 as being possible hair-growthmodulating protein biomarkers. [BMB reports 2012; 45(4):253-258]

Keywords

• Dermal fibroblast
• Dermal papilla cell
• Extracellular matrix
• Proteome
• Secretome