Bio-Protocol (Dec 2016)

Lymphocyte Isolation, Th17 Cell Differentiation, Activation, and Staining

  • Pawan Kumar,
  • Jay Kolls

DOI
https://doi.org/10.21769/BioProtoc.2047
Journal volume & issue
Vol. 6, no. 23

Abstract

Read online

In vitro Th17 (α, β T helper cell which produce IL-17A, IL-17F and IL-22) differentiation has been routinely used for functional T cells studies. Here we describe a method for Th17 cell differentiation.