Development and validation of a stability indicating green analytical method for the simultaneous estimation of l-glutathione, n-acetyl l-cysteine and Vitamin C in marketed formulation using UV–visible spectroscopy

Abstract

Abstract Background An efficient ultraviolet spectrophotometric method has been devised for the simultaneous analysis of l-glutathione, n-acetyl l-cysteine and Vitamin C in combined tablet dosage forms. This method is characterized by its simplicity, rapidity, precision, accuracy and cost-effectiveness. It employs the simultaneous equation method to determine the concentrations of l-glutathione, n-acetyl l-cysteine and Vitamin C. Results The concentration range of 50–250 μg/mL for l-glutathione, 15–75 μg/mL for n-acetyl l-cysteine and 2–10 μg/mL for Vitamin C demonstrated linearity in this study. The simultaneous equation method was employed to determine the drug concentrations. The average recovery rates were found to be 99.94 ± 0.61% for l-glutathione, 100.90 ± 0.96 for n-acetyl l-cysteine and 99.81 ± 0.58% for Vitamin C. Stability studies were performed under various stress conditions. Conclusion The method employed in this study was observed to possess qualities of simplicity, accuracy and precision, making it suitable for the simultaneous determination of l-glutathione, n-acetyl l-cysteine and Vitamin C in pharmaceutical tablet dosage forms. The analysis results were statistically validated and supported by recovery studies and stability studies, further enhancing the credibility of the method.

Keywords

• Distilled water
• l-glutathione
• n-acetyl l-cysteine
• Vitamin C
• Validation
• Simultaneous equations