The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy

Shayne E. Quinn; Lu Huang; Jason G. Kerkvliet; Joel A. Swanson; Steve Smith; Adam D. Hoppe; Robert B. Anderson; Natalie W. Thiex; Brandon L. Scott

doi:10.1038/s41467-021-25187-1

Nature Communications (Aug 2021)

The structural dynamics of macropinosome formation and PI3-kinase-mediated sealing revealed by lattice light sheet microscopy

Shayne E. Quinn,
Lu Huang,
Jason G. Kerkvliet,
Joel A. Swanson,
Steve Smith,
Adam D. Hoppe,
Robert B. Anderson,
Natalie W. Thiex,
Brandon L. Scott

Affiliations

Shayne E. Quinn: Nanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)
Lu Huang: Department of Biology and Microbiology, South Dakota State University (SDSU)
Jason G. Kerkvliet: BioSNTR, SDSU
Joel A. Swanson: Department of Microbiology and Immunology, University of Michigan
Steve Smith: Nanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)
Adam D. Hoppe: BioSNTR, SDSU
Robert B. Anderson: Nanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)
Natalie W. Thiex: Department of Biology and Microbiology, South Dakota State University (SDSU)
Brandon L. Scott: Nanoscience and Nanoengineering, South Dakota School of Mines and Technology (South Dakota Mines)

DOI: https://doi.org/10.1038/s41467-021-25187-1
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 12

Abstract

Read online

Macropinocytosis is a cellular process for the uptake of extracellular fluid. Here, the authors use lattice light sheet microscopy to examine the spatial dynamics of the plasma membrane, PI3K activity, and structural differences of various macrophage cell types during macropinocytosis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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