Antibiotics (May 2022)

Epidemiology of Plasmids in <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> with Acquired Extended Spectrum Beta-Lactamase Genes Isolated from Chronic Wounds in Ghana

  • Frederik Pankok,
  • Stefan Taudien,
  • Denise Dekker,
  • Thorsten Thye,
  • Kwabena Oppong,
  • Charity Wiafe Akenten,
  • Maike Lamshöft,
  • Anna Jaeger,
  • Martin Kaase,
  • Simone Scheithauer,
  • Konstantin Tanida,
  • Hagen Frickmann,
  • Jürgen May,
  • Ulrike Loderstädt

DOI
https://doi.org/10.3390/antibiotics11050689
Journal volume & issue
Vol. 11, no. 5
p. 689

Abstract

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Little information is available on the local epidemiology of mobile genetic elements such as plasmids harboring acquired beta-lactamase genes in Western African Ghana. In the present study, we screened for plasmids in three Escherichia coli and four Klebsiella pneumoniae isolates expressing extended spectrum beta-lactamases (ESBL) mediated by the blaCTX-M-15 gene from chronically infected wounds of Ghanaian patients. Bacterial isolates were subjected to combined short-read and long-read sequencing to obtain the sequences of their respective plasmids. In the blaCTX-M-15-gene-carrying plasmids of the four ESBL-positive K. pneumoniae isolates, IncFIB/IncFII (n = 3) and FIA (n = 1) sequences were detected, while in the blaCTX-M-15-gene-carrying plasmids of the three ESBL-positive E. coli isolates, IncFIA/IncFIB (n = 2) and IncFIB (n = 1) sequences were found. The three IncFIB/IncFII sequence-containing plasmids were almost identical to a K. pneumoniae plasmid reported from France. They belonged to the clonal lineages ST17, ST36 and ST39 of K. pneumoniae, suggesting transversal spread of this obviously evolutionary successful plasmid in Ghana. Other resistance gene-encoding plasmids observed in the assessed Enterobacterales harbored IncFIA/IncR and IncFII sequences. International spread was confirmed by the high genetic similarity to resistance-mediating plasmids published from Asia, Australia, Europe and Northern America, including a blaCTX-M-15-gene-carrying plasmid isolated from a wild bird in Germany. In conclusion, the study contributed to the scarcely available information on the epidemiology of third-generation cephalosporine resistance-mediating plasmids in Ghana. Furthermore, the global spread of resistance-mediating plasmids provided hints on the evolutionary success of individual resistance-harboring plasmids by transversal spread among K. pneumoniae lineages in Ghana.

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