Evaluation of Antibacterial Activity and Cytotoxicity of Green Synthesized Silver Nanoparticles Using Scoparia Dulcis

Abstract

Green synthesis of silver nanoparticles (AgNPs) is gaining momentum in the field of nano-research. Scoparia dulcis leaves were used as a reducing agent for the synthesis of silver nanoparticles from an aqueous solution of silver nitrate (AgNO3). Synthesized AgNPs were characterized by UV-Vis spectroscopy, XRD, SEM with EDAX and TEM. UV-VIS surface plasmon resonance spectroscopy was observed at 430 nm. XRD data depicts that the NPs are crystalline in nature. The EDAX data indicate that 63.76% presence of Ag metal. The TEM & SEM results indicate that size of the AgNPs ranges from 15-25 nm. The results also support that spherical shape of the nanoparticles. In addition, the NPs are in polydispersed condition. The antimicrobial activities indicate significant inhibition of the growth of three pathogenic bacteria such as Pseudomonas ariginosa, Bacillua subtillis and Staphylococcus aureous. Cytotoxicity of this nanoparticle showed that this Ag-NP also has more cytotoxic effect on a lung cancer cell line, A549 cells compared to ovarian cancer cell line, PA1 indicating a possible therapeutic use of this AgNP.

Keywords

• silver nanoparticles
• green silver nanoperticle synthesis
• plant extract
• antimicrobial activity
• cytotoxicity assay