Early LPS-induced ERK activation in retinal pigment epithelium cells is dependent on PIP2-PLC

Abstract

This article presents additional data regarding the study “The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium” [1]. The new data presented here show that short exposure of RPE cells to lipopolysaccharide (LPS) induces an early and transient activation of the extracellular signal-regulated kinase (ERK1/2). This early ERK1/2 activation is dependent on phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC). On the contrary, neither the phospholipase D 1 (PLD1) nor the PLD2 inhibition is able to modulate the early ERK1/2 activation induced by LPS in RPE cells. Keywords: Retinal pigment epithelium (RPE), Inflammation, Phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC), Phospholipase D (PLD), Extracellular signal-regulated kinase (ERK1/2), Lipopolysaccharide (LPS)