First Karyotypic Study of the Kissing Gouramies, Helostoma temminckii (Anabantiformes, Helostomatidae) from Thailand by Conventional and Ag-NOR staining techniques

Abstract

The first chromosomal study of kissing Gourami, Helostoma temminckii from Thailand was karyotypically performed. Kidney cells were obtained from ten male and ten female specimens. Chromosomes were conventionally stained, and Ag-NOR banding was carried out. Conventional staining mainly demonstrated telocentric chromosome with diploid number (2n) and fundamental number (NF) as 48. The Ag-NOR banding technique showed the position of nucleolar organizer regions (NORs) at the end of the telomeres of chromosome pair four. The karyotype consisted of 16 large, 30 medium and two small telocentric chromosomes. Thus, the karyotype formula can be represented as follows: Lt16+Mt30+St2.

Keywords

• helostoma temminckii
• karyotype
• idiogram
• chromosome