THE SUCCESS RATE OF ARTIFICIAL INSEMINATION USING LIQUID SEMEN WITH DIFFERENT DILUENTS IN BALI CATTLE

Abstract

Artificial insemination using liquid semen is beneficial for areas where liquid nitrogen is not available. The purpose of this study was to determine the success rate of artificial insemination using liquid semen with different diluents based on non-return rate (NRR) and conception rate (CR). Research location is at BPTU-HPT Denpasar Breeding Center Pulukan. The material used in this study is Bali cattle which have a Body Condition score (BCS) of 2.5-3 (scale 1-5), physically fit, not pregnant, no reproductive disorders. The method used in this study was experimental field 3 treatments and 20 replications. The number of cows used was 60, each of which was treated 20 cows: PO = AI using andromed diluent , P 1 = AI using Tris aminomethane diluent + 20% egg yolk, P2 = AI using CEP-3 + 20% egg yolk diluent . Before conducting AI cows in estrus synchronization using PGF2α (Enzaprost -T) then in AI using liquid semen that is stored 1-3 days with an average motility of each treatment P0, P1, P2: 57 ± 6,19; 60 ± 5,65; 57 ± 5,63 . AI uses a double dose at the second and eighth hour after estrus. The results of each treatment P0, P1 and P2 are NRR1: 80%, 60%, 50%; CR = 45%, 40%, 35%. The conclusion of this study is that diluted andromed (P0) has a higher success rate when compared to P1 and P2.

Keywords

• artificial insemination
• liquid semen
• non-return rate
• conception rate