Isolation and identification of a high-efficiency phenol-degrading bacteria and optimization of its degradation conditions

Abstract

Phenol is widely used in China, it not only pollutes the environment, but also accumulates toxic substances in the human body through the food chain, further harming humans. In this experiment, a strain of high-efficiency low-temperature degradation phenol bacteria B5 was selected from the soil contaminated by organic matter of Lanzhou. Through research methods such as Gram staining observation, DNA extraction, PCR amplification, sequencing and comparison, it was found that this strain was Pantoea agglomerans. Through the subsequent optimization of degradation conditions, it was found that the B5 strain can degrade 500mg/L of phenol to 24.8mg/L in 36h. The ability to degrade phenol is stronger between pH5.5-pH6.0, and the ability to degrade phenol is higher in a medium containing 4-8g/L sodium chloride. This research can provide certain theoretical guidance for phenol degradation.