Fungal Communities Associated with Corn in a Diverse Long-Term Crop Rotation in Ohio

Abstract

Crop rotation is a cultural practice in disease management used to break the disease cycle, resulting in a reduction of inoculum. In Ohio, crop rotations have been reduced in diversity, with many farmers shifting to corn-soy rotations from more diverse rotations featuring wheat. We investigated the impact of this shift on soil fungal communities under corn by conducting synthetic long-read amplicon sequencing. DNA was extracted from soil sampled during the corn-growing season at two locations in Ohio with replicated long-term rotation plots in a corn–soybean (CS) and corn–soybean–winter wheat (CSW) rotation in 2018, 2019, and 2020. 18s-ITS amplicons were sequenced using Illumina paired with Loopgenomics technology. PERMANOVA analysis revealed that fungal communities were significantly impacted by location, rotation, and time. Shannon and Simpson diversity indices implied fungal species diversity was significantly higher in the CS rotation. Indicator species analysis revealed 20 species indicative of the CSW rotation, including Mortierella, Hymenoschyphus, Ascobolus, and Saitozyma species, and 36 species indicative of the CS rotation, including Fusarium, Neoaschocyta, Zalerion, and Trichoderma species, among others. Indicator species amplicon sequencing variant reads for the CSW rotation were not correlated with the decline in corn yield or soil nitrogen, carbon, or active carbon. However, they were positively correlated with soil organic matter. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.

Keywords

• big data
• bioinformatics
• ecology
• fungal pathogens
• microbiome
• systems biology