Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry

Danye Qiu; Miranda S. Wilson; Verena B. Eisenbeis; Robert K. Harmel; Esther Riemer; Thomas M. Haas; Christopher Wittwer; Nikolaus Jork; Chunfang Gu; Stephen B. Shears; Gabriel Schaaf; Bernd Kammerer; Dorothea Fiedler; Adolfo Saiardi; Henning J. Jessen

doi:10.1038/s41467-020-19928-x

Nature Communications (Nov 2020)

Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry

Danye Qiu,
Miranda S. Wilson,
Verena B. Eisenbeis,
Robert K. Harmel,
Esther Riemer,
Thomas M. Haas,
Christopher Wittwer,
Nikolaus Jork,
Chunfang Gu,
Stephen B. Shears,
Gabriel Schaaf,
Bernd Kammerer,
Dorothea Fiedler,
Adolfo Saiardi,
Henning J. Jessen

Affiliations

Danye Qiu: Institute of Organic Chemistry, University of Freiburg
Miranda S. Wilson: Medical Research Council, Laboratory for Molecular Cell Biology, University College London
Verena B. Eisenbeis: Institute of Organic Chemistry, University of Freiburg
Robert K. Harmel: Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Esther Riemer: Institute of Crop Science and Resource Conservation, Department of Plant Nutrition, Rheinische Friedrich-Wilhelms-University Bonn
Thomas M. Haas: Institute of Organic Chemistry, University of Freiburg
Christopher Wittwer: Institute of Organic Chemistry, University of Freiburg
Nikolaus Jork: Institute of Organic Chemistry, University of Freiburg
Chunfang Gu: Signal Transduction Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health
Stephen B. Shears: Signal Transduction Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health
Gabriel Schaaf: Institute of Crop Science and Resource Conservation, Department of Plant Nutrition, Rheinische Friedrich-Wilhelms-University Bonn
Bernd Kammerer: Institute of Organic Chemistry, University of Freiburg
Dorothea Fiedler: Leibniz-Forschungsinstitut für Molekulare Pharmakologie
Adolfo Saiardi: Medical Research Council, Laboratory for Molecular Cell Biology, University College London
Henning J. Jessen: Institute of Organic Chemistry, University of Freiburg

DOI: https://doi.org/10.1038/s41467-020-19928-x
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 12

Abstract

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Myo-Inositol phosphates (InsPs) and pyrophosphates (PP-InsPs) are important second messengers but their analysis remains challenging. Here, the authors develop a capillary electrophoresis-mass spectrometry method for the identification and quantitation of InsP and PP-InsP isomers in cells and tissues.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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