Fast Detection of Choline Containing Compounds in Dairy Products Using NMR

Abstract

Choline containing compounds (CCCs) are a class of small molecule metabolites with important biological functions. Some CCCs can be synthesized by human body, but most of them come from daily diet. CCCs exist in milk, beans, and infant formulas additives. Chronic choline deficiency in the diet is associated with diseases such as organ dysfunction. Therefore, fast detection for CCCs is important for food research. Nuclear magnetic resonance (NMR) spectroscopy is a noninvasive method for CCCs measurement. In this study, 1H-14N SOFAST-HMQC was used to measure the CCCs in dairy products. It was observed that CCCs detection by this method can be more selective than traditional one-dimensional 1H NMR, and the detection sensitivity per unit time was 1.5~2.0 times higher than that of the previous developed 1H-14N HSQC method.

Keywords

• liquid NMR
• 1H-14N SOFAST-HMQC
• choline
• dairy products