Integrated multi-omics reveals the relationship between growth performance, rumen microbes and metabolic status of Hu sheep with different residual feed intakes

Yanzhen Zhang; Xiaowei Zhang; Dingren Cao; Jinyong Yang; Huiling Mao; Lingling Sun; Chong Wang

Animal Nutrition (Sep 2024)

Integrated multi-omics reveals the relationship between growth performance, rumen microbes and metabolic status of Hu sheep with different residual feed intakes

Yanzhen Zhang,
Xiaowei Zhang,
Dingren Cao,
Jinyong Yang,
Huiling Mao,
Lingling Sun,
Chong Wang

Affiliations

Yanzhen Zhang: College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China; Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, China
Xiaowei Zhang: Zhejiang Provincial Animal Husbandry Technology Promotion and Monitoring Station of Breeding Livestock and Poultry, Hangzhou 310021, China
Dingren Cao: Zhejiang Provincial Animal Husbandry Technology Promotion and Monitoring Station of Breeding Livestock and Poultry, Hangzhou 310021, China
Jinyong Yang: Zhejiang Provincial Animal Husbandry Technology Promotion and Monitoring Station of Breeding Livestock and Poultry, Hangzhou 310021, China
Huiling Mao: College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China; Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, China
Lingling Sun: College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China; Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, China; Department of Animal Science, Shandong Vocational Animal Science and Veterinary College, Weifang 261061, China; Corresponding authors.
Chong Wang: College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, China; Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou 311300, China; Corresponding authors.

Journal volume & issue: Vol. 18
pp. 284 – 295

Abstract

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Residual feed intake (RFI) is a metric that provides a more accurate measure of feed efficiency. The lower the RFI, the higher the feed efficiency. The changes in the host microbiome and metabolome contribute to the greater feed efficiency of low RFI (LRFI) animals. The aim of this study was to explore the differences in rumen microorganisms, rumen metabolites and plasma metabolites of Hu sheep with differing RFI through the microbiome and metabolome. A total of 80 Hu sheep were used. The experiment consisted of a 15-d pretrial period and a 128-d experimental period. The RFI in the experimental period was calculated for all sheep, and the sheep were screened into high RFI (HRFI, n = 8) and LRFI (n = 8) groups. The HRFI and LRFI sheep did not differ in their initial and final body weights, average daily gain and body measurements, but the dry matter intake of LRFI sheep was significantly decreased (28.4%, P < 0.001). The sheep with LRFI had higher digestibility of crude protein (P = 0.010) and ether extract (P = 0.010) compared to HRFI group. The concentrations of acetate (P = 0.036), propionate (P = 0.010), valerate (P = 0.027) and total volatile fatty acids (P = 0.048) in rumen of LRFI group were higher compared to HRFI group. The results of 16S rDNA sequencing indicated that the sheep with LRFI had higher proportions of Prevotella genus in rumen liquid (P = 0.031). The rumen metabolome and plasma metabolome results showed that the citrate cycle, pyruvate metabolism and alanine, aspartate and glutamate metabolism processes were more active for sheep in LRFI group, which provided more energy substrate such as malic acid, oxoglutaric acid and citric acid. In conclusion, sheep with LRFI can utilize feed more efficiently, and the more active energy metabolism pathway and the production of energy substances may account for the higher feed efficiency.

Published in Animal Nutrition

ISSN: 2405-6545 (Print)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Animal culture
Website: https://www.keaipublishing.com/en/journals/animal-nutrition/

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