Аграрная наука Евро-Северо-Востока (Aug 2017)
Study of chemical composition and toxic properties of phytocomplex containing biologically active substances
Abstract
The aim of the research was to study the chemical composition and toxic properties of plant phytocomplex, which have the most pronounced adaptogenic properties. Object of the study: a phytocomplex including levsea safflower (Rhaponticum carthamoides), serpent crown (Serratula coronata), fenugreev creeper (Filipendula ulmaria). Initially, the chemical composition was determined in selected plants, and then the alcohol extract from the phytocomplex was tested in white mice for toxicity. The toxicity study of extract of the studied phytocomplex was carried out on mice divided into 2 experiment and 2 control groups (n = 5 in each group), which was injected parenterally and intragastrically at once in a volume of 0.5 ml/mouse; the maximum dose of extractive substances of the phytocomplex was 2.5 g/kg of body weight in terms of dry matter. In the control, physiological saline was injected once in the same volume. Within 10 days the clinical condition of the animals was monitored. Before and after the experiment the blood was examined for the number of erythrocytes, leukocytes and hemoglobin concentration. The results of studies of phytocomplex containing water-alcohol extracts of the herbs Rhaponticum carthamoides, Serratula coronata, Filipendula ulmaria, showed that the biologically active substances in it are: amino acids - aspartic (3.5.3.9 g/100 g), glutamic (2,5...3.0 g /100 g), leucine (1.9...2.0 g/100 g), tyrosine (1.2...1.4 g/100 g), lysine (1.6 g /100 g ); Vitamins C (62.0.77.0 mg /%), E (6.2 mg /%), carotenoids (65.0.113.0 mg /%), PP (11.5 mg /% ); Ecdysteroids (418.0.2170 mg / kg), and flavonoids (3.0.. .7.2% of the dry matter). All the herbs of the phytocomplex contain protein, sugars, vitamins, amino acids, tannins and flavonoids. Intragastric and intraperitoneal administration of the phytocomplex to white mice showed no toxic properties.
