Non-CG DNA methylation is a biomarker for assessing endodermal differentiation capacity in pluripotent stem cells

Lee M. Butcher; Mitsuteru Ito; Minodora Brimpari; Tiffany J. Morris; Filipa A. C. Soares; Lars Ährlund-Richter; Nessa Carey; Ludovic Vallier; Anne C. Ferguson-Smith; Stephan Beck

doi:10.1038/ncomms10458

Nature Communications (Jan 2016)

Non-CG DNA methylation is a biomarker for assessing endodermal differentiation capacity in pluripotent stem cells

Lee M. Butcher,
Mitsuteru Ito,
Minodora Brimpari,
Tiffany J. Morris,
Filipa A. C. Soares,
Lars Ährlund-Richter,
Nessa Carey,
Ludovic Vallier,
Anne C. Ferguson-Smith,
Stephan Beck

Affiliations

Lee M. Butcher: UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK
Mitsuteru Ito: Department of Genetics, University of Cambridge
Minodora Brimpari: Department of Surgery, Anne McLaren Laboratory, Wellcome Trust and Medical Research Council Stem Cell Institute, University of Cambridge
Tiffany J. Morris: UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK
Filipa A. C. Soares: Department of Surgery, Anne McLaren Laboratory, Wellcome Trust and Medical Research Council Stem Cell Institute, University of Cambridge
Lars Ährlund-Richter: Division of Paediatric Oncology, Department of Women’s and Children’s Health, Karolinska Institutet
Nessa Carey: PraxisUnico, The Jeffreys Building, St John’s Innovation Park
Ludovic Vallier: Department of Surgery, Anne McLaren Laboratory, Wellcome Trust and Medical Research Council Stem Cell Institute, University of Cambridge
Anne C. Ferguson-Smith: Department of Genetics, University of Cambridge
Stephan Beck: UCL Cancer Institute, University College London, 72 Huntley Street, London WC1E 6BT, UK

DOI: https://doi.org/10.1038/ncomms10458
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 8

Abstract

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The methylation of non-CpG residues is a poorly understood marker of pluripotent cells, gradually lost as cells differentiate. Here the authors show non-CG methylation can be used as a marker of differentiation potential.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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