Mass Spectrometry Strategies for <i>O</i>-Glycoproteomics

Abstract

Glycoproteomics has accelerated in recent decades owing to numerous innovations in the analytical workflow. In particular, new mass spectrometry strategies have contributed to inroads in O-glycoproteomics, a field that lags behind N-glycoproteomics due to several unique challenges associated with the complexity of O-glycosylation. This review will focus on progress in sample preparation, enrichment strategies, and MS/MS techniques for the identification and characterization of O-glycoproteins.

Keywords

• glycoproteomics
• tandem mass spectrometry
• glycoprotease
• enrichment
• glycans
• analytical workflow