Nature Communications (Feb 2020)

Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression

  • Anna S. E. Cuomo,
  • Daniel D. Seaton,
  • Davis J. McCarthy,
  • Iker Martinez,
  • Marc Jan Bonder,
  • Jose Garcia-Bernardo,
  • Shradha Amatya,
  • Pedro Madrigal,
  • Abigail Isaacson,
  • Florian Buettner,
  • Andrew Knights,
  • Kedar Nath Natarajan,
  • HipSci Consortium,
  • Ludovic Vallier,
  • John C. Marioni,
  • Mariya Chhatriwala,
  • Oliver Stegle

DOI
https://doi.org/10.1038/s41467-020-14457-z
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 14

Abstract

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Studying the genetic effects on early stages of human development is challenging due to a scarcity of biological material. Here, the authors utilise induced pluripotent stem cells from 125 donors to track gene expression changes and expression quantitative trait loci at single cell resolution during in vitro endoderm differentiation.