Automated multiplex genome-scale engineering in yeast

Tong Si; Ran Chao; Yuhao Min; Yuying Wu; Wen Ren; Huimin Zhao

doi:10.1038/ncomms15187

Nature Communications (May 2017)

Automated multiplex genome-scale engineering in yeast

Tong Si,
Ran Chao,
Yuhao Min,
Yuying Wu,
Wen Ren,
Huimin Zhao

Affiliations

Tong Si: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
Ran Chao: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
Yuhao Min: Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
Yuying Wu: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
Wen Ren: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA
Huimin Zhao: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA

DOI: https://doi.org/10.1038/ncomms15187
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 12

Abstract

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Genome-scale engineering is a powerful technique for understanding biology and designing microorganisms but has been limited to bacterial species. Here the authors present an automated platform for genome-scale engineering inSaccharomyces cerevisiaeusing CRISPR-Cas and RNAi.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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