Genomic Resource Insights into Strain WZ-12: An Antimicrobial-Producing Streptomyces Isolate from Star Anise Rhizosphere Soil

Abstract

Strain WZ-12, isolated from the rhizosphere soil of star anise trees, exhibits significant antifungal efficacy against a variety of fungi, including Colletotrichum horii, the pathogenic fungus responsible for anthracnose in star anise. Based on 16S rRNA gene sequencing and phylogenetic analysis, the strain was identified as a member of the Streptomyces genus. The genome of WZ-12 comprises a linear chromosome of 9,169,400 bp and a plasmid of 378,325 bp, possessing a G + C content of 71.12%. Of note, the genome encompasses 8,172 protein-coding genes, 21 rRNA genes, 74 tRNA genes, and 89 other noncoding RNA genes. The COG, Swiss-Prot, GO, KEGG, and NR databases annotated up to 79.17, 50.50, 13.17, 37.51, and 90.43% of potential genes, respectively. Remarkably, the genome hosts 175 antimicrobial resistance genes and 33 secondary metabolite gene clusters that likely contribute to the biosynthesis of diverse bioactive compounds such as antibiotics and agents with antitumor, antioxidant, immunosuppressive, anti-inflammatory, or stress tolerance properties. These findings highlight the potential of WZ-12 as a promising source of novel bioactive molecules and suggest its significant value as a resource for developing innovative antimicrobial agents. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Keywords

• anthracnose
• complete genome
• rhizosphere soil
• star anise
• Streptomyces