Monoclonal antibodies binding data for SARS-CoV-2 proteins

Nawneet Mishra; Joan Teyra; RuthMabel Boytz; Shane Miersch; Trudy N. Merritt; Lia Cardarelli; Maryna Gorelik; Filip Mihalic; Per Jemth; Robert A. Davey; Sachdev S. Sidhu; Daisy W. Leung; Gaya K. Amarasinghe

Data in Brief (Aug 2022)

Monoclonal antibodies binding data for SARS-CoV-2 proteins

Nawneet Mishra,
Joan Teyra,
RuthMabel Boytz,
Shane Miersch,
Trudy N. Merritt,
Lia Cardarelli,
Maryna Gorelik,
Filip Mihalic,
Per Jemth,
Robert A. Davey,
Sachdev S. Sidhu,
Daisy W. Leung,
Gaya K. Amarasinghe

Affiliations

Nawneet Mishra: Department of Pathology and Immunology, Washington University School of Medicine in St Louis, St Louis, MO 63110, U.S.A.
Joan Teyra: The Donnelly Centre, University of Toronto, Toronto, Canada
RuthMabel Boytz: Department of Microbiology, Boston University School of Medicine, St Louis, MO 63110, U.S.A.
Shane Miersch: The Donnelly Centre, University of Toronto, Toronto, Canada
Trudy N. Merritt: Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, U.S.A.
Lia Cardarelli: The Donnelly Centre, University of Toronto, Toronto, Canada
Maryna Gorelik: The Donnelly Centre, University of Toronto, Toronto, Canada
Filip Mihalic: Department of Medical Biochemistry and Microbiology, Uppsala University, B.M.C. Box 582, Husargatan 3, 751 23 Uppsala Sweden
Per Jemth: Department of Medical Biochemistry and Microbiology, Uppsala University, B.M.C. Box 582, Husargatan 3, 751 23 Uppsala Sweden
Robert A. Davey: Department of Microbiology, Boston University School of Medicine, St Louis, MO 63110, U.S.A.
Sachdev S. Sidhu: The Donnelly Centre, University of Toronto, Toronto, Canada; Corresponding author.
Daisy W. Leung: Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, U.S.A.; Corresponding author.
Gaya K. Amarasinghe: Department of Pathology and Immunology, Washington University School of Medicine in St Louis, St Louis, MO 63110, U.S.A.; Corresponding author.

Journal volume & issue: Vol. 43
p. 108415

Abstract

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SARS-CoV-2 pandemic opens up the curiosity of understanding the coronavirus. This demand for the development of the regent, which can be used for academic and therapeutic applications. The present data provide the biochemical characterization of synthetically developed monoclonal antibodies for the SARS-CoV-2 proteins. The antibodies from phage-displayed antibody libraries were selected with the SARS-CoV-2 proteins immobilized in microwell plates. The clones which bind to the antigen in Fab-phage ELISA were selected, and a two-point competitive phage ELISA was performed. Antibodies binding kinetic of IgGs for SARS-CoV2 proteins further carried with B.L.I. Systematic analysis of binding with different control proteins and purified SARS-CoV-2 ensured the robustness of the antibodies.

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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