Nature Communications (Sep 2019)
Improving mass spectrometry analysis of protein structures with arginine-selective chemical cross-linkers
- Alexander X. Jones,
- Yong Cao,
- Yu-Liang Tang,
- Jian-Hua Wang,
- Yue-He Ding,
- Hui Tan,
- Zhen-Lin Chen,
- Run-Qian Fang,
- Jili Yin,
- Rong-Chang Chen,
- Xing Zhu,
- Yang She,
- Niu Huang,
- Feng Shao,
- Keqiong Ye,
- Rui-Xiang Sun,
- Si-Min He,
- Xiaoguang Lei,
- Meng-Qiu Dong
Affiliations
- Alexander X. Jones
- Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University
- Yong Cao
- School of Life Sciences, Peking University
- Yu-Liang Tang
- Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University
- Jian-Hua Wang
- National Institute of Biological Sciences (NIBS)
- Yue-He Ding
- National Institute of Biological Sciences (NIBS)
- Hui Tan
- Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University
- Zhen-Lin Chen
- Key Lab of Intelligent Information Processing, Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Run-Qian Fang
- Key Lab of Intelligent Information Processing, Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Jili Yin
- Key Lab of Intelligent Information Processing, Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Rong-Chang Chen
- University of Chinese Academy of Sciences
- Xing Zhu
- University of Chinese Academy of Sciences
- Yang She
- National Institute of Biological Sciences (NIBS)
- Niu Huang
- National Institute of Biological Sciences (NIBS)
- Feng Shao
- National Institute of Biological Sciences (NIBS)
- Keqiong Ye
- University of Chinese Academy of Sciences
- Rui-Xiang Sun
- National Institute of Biological Sciences (NIBS)
- Si-Min He
- Key Lab of Intelligent Information Processing, Chinese Academy of Sciences (CAS), Institute of Computing Technology, CAS
- Xiaoguang Lei
- Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University
- Meng-Qiu Dong
- National Institute of Biological Sciences (NIBS)
- DOI
- https://doi.org/10.1038/s41467-019-11917-z
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 11
Abstract
Cross-linking mass spectrometry can provide insights into protein structures and interactions but its scope depends on the reactivity of the cross-linker. Here, the authors develop Arg-Arg and Lys-Arg cross-linkers, which provide structural information elusive to the widely used Lys-Lys cross-linkers.
