Transcriptomic profiling of single circulating tumor cells provides insight into human metastatic gastric cancer

Ryo Negishi; Hitomi Yamakawa; Takeru Kobayashi; Mayuko Horikawa; Tatsu Shimoyama; Fumiaki Koizumi; Takeshi Sawada; Keisuke Oboki; Yasushi Omuro; Chikako Funasaka; Akihiko Kageyama; Yusuke Kanemasa; Tsuyoshi Tanaka; Tadashi Matsunaga; Tomoko Yoshino

doi:10.1038/s42003-021-02937-x

Communications Biology (Jan 2022)

Transcriptomic profiling of single circulating tumor cells provides insight into human metastatic gastric cancer

Ryo Negishi,
Hitomi Yamakawa,
Takeru Kobayashi,
Mayuko Horikawa,
Tatsu Shimoyama,
Fumiaki Koizumi,
Takeshi Sawada,
Keisuke Oboki,
Yasushi Omuro,
Chikako Funasaka,
Akihiko Kageyama,
Yusuke Kanemasa,
Tsuyoshi Tanaka,
Tadashi Matsunaga,
Tomoko Yoshino

Affiliations

Ryo Negishi: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology
Hitomi Yamakawa: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology
Takeru Kobayashi: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology
Mayuko Horikawa: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology
Tatsu Shimoyama: Department of Medical Oncology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
Fumiaki Koizumi: Department of Laboratory Medicine, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
Takeshi Sawada: Department of Medical Oncology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
Keisuke Oboki: Center for Medical Research Cooperation, Tokyo Metropolitan Institute of Medical Science
Yasushi Omuro: Department of Medical Oncology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
Chikako Funasaka: Department of Medical Oncology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
Akihiko Kageyama: Department of Medical Oncology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
Yusuke Kanemasa: Department of Medical Oncology, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
Tsuyoshi Tanaka: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology
Tadashi Matsunaga: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology
Tomoko Yoshino: Division of Biotechnology and Life science, Institute of Engineering, Tokyo University of Agriculture and Technology

DOI: https://doi.org/10.1038/s42003-021-02937-x
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

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Ryo Negishi et al. utilize a size-dependent recovery method with gel-based cell manipulation to isolate single circulating tumor cells (CTCs) for transcriptomic analysis. They demonstrate the utility of this approach by analyzing the transcriptomic profile of CTCs from human gastric cancer patients.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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