Journal of Applied Biomaterials & Functional Materials (Mar 2021)

Study on proanthocyanidins crosslinked collagen membrane for guided bone tissue regeneration

  • Hongfa Yang,
  • Wai-Ching Liu,
  • Xinrui Liu,
  • Yunqian Li,
  • Chingpo Lin,
  • Yu-Min Lin,
  • An-Ni Wang,
  • Thu-Trang Nguyen,
  • Yu-Chien Lin,
  • Ren-Jei Chung

DOI
https://doi.org/10.1177/22808000211005379
Journal volume & issue
Vol. 19

Abstract

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The goal of this study is to understand the ability of a newly developed barrier membrane to enhance bone tissue regeneration. Here in this study we present the in vitro characterization of the barrier membrane made from type I collagen and crosslinked by oligomeric proanthocyanidins (OPCs). The effects of the membrane (P-C film) on cell cycle, proliferation, alkaline phosphatase activity, and mineralization were evaluated using the human osteoblast cell line MG-63, while the barrier ability was examined using MG-63 cells, as well as the human skin fibroblast cell line WS-1. The pore size is one of the factors that plays a key role in tissue regeneration, therefore, we evaluated the pore size of the membrane using a capillary flow porometer. Our results showed that the mean pore size of the P-C film was approximately 7–9 µm, the size known to inhibit cell migration across the membrane. The P-C film also demonstrated excellent cell viability and good biocompatibility, since the cell number increased with time, with MG-63 cells proliferating faster on the P-C film than in the cell culture flask. Furthermore, the P-C film promoted osteoblast differentiation, resulting in higher alkaline phosphatase activity and mineralization. Therefore, our results suggest that this P-C film has a great potential to be used in guided bone regeneration during periodontal regeneration and bone tissue engineering.