Examination of Bone Marrow Mesenchymal Stem Cells Seeded onto Poly(3-hydroxybutyrate-co-3-hydroxybutyrate) Biological Materials for Myocardial Patch

Abstract

The implantation of the Bone Marrow Mesenchymal Stem Cells (BMSCs) into the heart has been reported to be effective for the treatment of myocardial infarction; however, the methods most suitable for supporting stem cell growth in a myocardial patch, still remain unknown. We used a new polymer material composed of poly(3-hydroxybutyrate-co-3-hydroxybutyrate) [P(3HB-co-3HB)] co-cultured with BMSCs to create a myocardial patch. The BMSCs were obtained from healthy male BSL-C57 mice. The cells were treated with 5-azacytidine to investigate their differentiation into cardiomyocytes. The cells were seeded for 24 hours onto the P(3HB-co-3HB) biological material films (n=8). Cell-biomaterial constructs were fixed and analyzed using different methods. BMSCs were CD34-, CD45-, CD90+ (low) and CD73+. The cells were stained with anti-cardiac troponin T (cTnT) and anti-connexin 43 (CX43) antibodies after 5-azacytidine treatment. Scanning electron microscopy revealed that the morphology of the BMSC was normal and that cell numbers were more abundant on the P(3HB-co-3HB) material surfaces. The growth curve of the BMSCs on the biomaterial patches showed the P(3HB-co-3HB) material enhanced good stem cell growth. Owing to its excellent biocompatibility and biodegradability properties, in particular its porosity, the P(3HB-co-3HB) is hailed as an optimal material to support myocardial cell growth and to create a myocardial patch in patients with myocardial infarction.

Keywords

• myocardial regeneration;
• bone marrow mesenchymal stem cell (MSC);
• myocardial infarction;
• poly(3-hydroxybutyrate-co-3-hydroxybutyrate) [P(3HB-co-3HB)].