Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva

Abstract

Nanolayer deposition(s) of Ag and AgC composite on a plastic substrate was used to design disposable stochastic sensors. Three shapes of nanocoatings were tested. The first shape was obtained by deposition of a nanofilm of Ag on the plastic material; the second shape was obtained by deposition of a composite AgC nanolayer on the plastic material; the third shape was obtained by nanolayer deposition of a composite material of AgC on the top of the Ag nanofilm deposited on the plastic material. α-Cyclodextrin was used to modify the active surface of the sensor. Wide linear concentration ranges were obtained as follows: for de-assay of α-amylase in whole blood: 1.00 × 10−7–1.00 × 103 U mL−1 and for the assay of α-amylase in saliva: 1.5 × 10−15–1.5 × 102 U mL−1. α-Amylase was reliably determined from whole blood and saliva samples using the proposed disposable stochastic sensors.

Keywords

• α-amylase
• disposable stochastic sensors
• nanolayer deposition(s)
• bioanalysis