ACE2 N-glycosylation modulates interactions with SARS-CoV-2 spike protein in a site-specific manner

Affiliations

Ayana Isobe: Department of Infectious Diseases, Kyoto Prefectural University of Medicine
Yasuha Arai: Department of Infectious Diseases, Kyoto Prefectural University of Medicine
Daisuke Kuroda: Research Center for Drug and Vaccine Development, National Institute of Infectious Diseases
Nobuaki Okumura: Institute for Protein Research, Osaka University
Takao Ono: SANKEN, Osaka University
Shota Ushiba: Murata Manufacturing Co., Ltd.
Shin-ichi Nakakita: Division of Functional Glycomics, Kagawa University
Tomo Daidoji: Department of Infectious Diseases, Kyoto Prefectural University of Medicine
Yasuo Suzuki: Department of Medical Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka
Takaaki Nakaya: Department of Infectious Diseases, Kyoto Prefectural University of Medicine
Kazuhiko Matsumoto: SANKEN, Osaka University
Yohei Watanabe: Department of Infectious Diseases, Kyoto Prefectural University of Medicine

Abstract

Three glycosylation sites on the cellular receptor for SARS-CoV-2, ACE2, are probed for interactions with the spike protein and cooperativity.