Memorias do Instituto Oswaldo Cruz (Aug 2011)

Sequence analysis of the 2009 pandemic influenza A H1N1 virus haemagglutinin gene from 2009-2010 Brazilian clinical samples

  • João Leandro de Paula Ferreira,
  • Samanta Etel Treiger Borborema,
  • Luis Fernando de Macedo Brígido,
  • Maria Isabel de Oliveira,
  • Terezinha Maria de Paiva,
  • Cecília Luiza Simões dos Santos

DOI
https://doi.org/10.1590/S0074-02762011000500014
Journal volume & issue
Vol. 106, no. 5
pp. 613 – 616

Abstract

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In this paper, we analysed the haemagglutinin (HA) gene identified by polymerase chain reaction from 90 influenza A H1N1 virus strains that circulated in Brazil from April 2009-June 2010. A World Health Organization sequencing protocol allowed us to identify amino acid mutations in the HA protein at positions S220T (71%), D239G/N/S (20%), Y247H (4.5%), E252K (3.3%), M274V (2.2%), Q310H (26.7%) and E391K (12%). A fatal outcome was associated with the D239G mutation (p < 0.0001). Brazilian HA genetic diversity, in comparison to a reference strain from California, highlights the role of influenza virus surveillance for study of viral evolution, in addition to monitoring the spread of the virus worldwide.

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