The correlation between structural changes in articular hyaline cartilage and the parameters of systemic manifestations of inflammatory reaction in the pathogenesis of early stages of primary osteoarthritis

Abstract

Aim: to study the correlations of structural disorders of articular hyaline cartilage with changes in concentration of pro- and anti-inflammatory cytokines at the early stages of osteoarthritis. Material and Methods. Ultrasound and MRI investigations of knee joints (T2*-relaxometry) were performed to 67 females of main group aged 35-50 with initial manifestations of (0-1 stages) of primary osteoarthritis and 30 conventionally healthy individuals. The level of interleukin-1 (3 was determined in blood serum with multi-parameter system MAGPIX (Luminex Corporation), Urine CTX-II was identified in urine and YKL- 40, interleukin-4 — in blood serum of the subjects by ELISA. Results. The levels of Urine CTX-II: 32.5 (30.9; 35.1) ng/ml, interleukin-1 (3: 6.09 (4.81; 7.69) pg/ml and YKL-4064.1 (58.1; 77.0) ng/ml of main group patients were higherthan in the comparison group. The correlation force between Urine CTX-II and cartilage glycoprotein YKL-40 (R=0.7); interleukin-1 (3 and cartilage glycoprotein YKL-40 (R=0.3) was lower; and the correlation force between YKL-40 and CTX-II (R=0.4 (p<0.05)) the connection was higher. Conclusion. Early stages of primary osteoarthritis are accompanied by degenerative changes inhyaline cartilages that is verified by T2*-relaxometry results, and increased excretion of Urine CTX-II on  the background of pro-inflammatory mediators hyperproduction, more significant increase in the concentration of cartilage glycoprotein YKL-40 compared to interleukin-1 (3 which comes together with the alteration of the system of correlations between the levels of pro-inflammatory cytokines and the parameters of degenerative dystrophic changes in articular cartilage. 

Keywords

• articular hyaline cartilage
• cytokines
• early stages
• osteoarthrosis