PREPARATION OF EXCRETORY SECRETORY PROTEIN FROM BRUGIAN MICROFILARIAE ISOLATED FROM CANINE BLOOD

Abstract

The article describes the culture of microfilaria of Brugia spp. for the extraction of excretory-secretory protein. The microfilariae were separated from the various components of canine blood using HiSep™ LSM by gradient centrifugation method. The microfilariae were cultured in RPMI-1640 media and incubated at 37°C. The spent media was collected at 12 hour intervals, filtered, pooled and stored at -50°C. Protein estimation was done by Bradford’s method. The excretory-secretory protein produced can further be used in diagnostic tests.

Keywords

• excretory secretory protein
• brugiasp. hisep
• canine