Describing a modern therapeutic drug prepared by in situ decorated gold nanoparticles on starch-modified magnetic nanoparticles to treat the cutaneous wound: a preclinical trial study

Abstract

In recent days, the green synthesized nanomagnetic biocomposites have been evolved with tremendous potential as the future catalysts. This has encouraged us to design and synthesis of a novel Au NP fabricated starch functionalized core-shell type nanomaterial (Fe3O4/Starch-Au nanocomposite). It was meticulously characterized using advanced analytical techniques like FT-IR, FESEM, TEM, EDX, VSM, XRD and ICP-OES. MTT assay was used on common normal cell line i.e. Human umbilical vein endothelial cells (HUVEC) to survey the cytotoxicity effects of Fe3O4/Starch-Au nanocomposite. In the part of cutaneous wound healing, use of Fe3O4/Starch-Au nanocomposite ointment, significantly (p ≤ 0.01) raised the wound contracture, vessel, hydroxyl proline, hexuronic acid, fibrocyte, fibroblast, and fibrocytes/fibroblast rate and significantly (p ≤ 0.01) decreased the wound area, total cells, neutrophil, and lymphocyte compared to other groups in rats. Finally, the results showed the useful cutaneous wound healing properties of Fe3O4/Starch-Au nanocomposite. Maybe significant cutaneous wound healing potentials of Fe3O4/Starch-Au nanocomposite are related to their antioxidant activities. For investigating the antioxidant activities of Fe3O4/Starch-Au nanocomposite, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. The Fe3O4/Starch-Au nanocomposite inhibited half of the DPPH molecules in the concentration of 156 µg/mL.

Keywords

• Fe3O4/Starch-Au
• cutaneous wound healing
• antioxidant
• magnetic composite