Expression of CD86 Co-stimulatory gene in colon polyps

Abstract

Introduction: Since the majority of colorectal cancers originate from polyps in the area, therefore, the investigation of the immunological gene expression in colon polyps can be used as a factor for the detection of genetic and pathological differences in polyps. The aim of this study was investigating the expression of CD86 gene in colorectal polyps and its association with malignancy. Materials and Methods: In a descriptive-analytical study during (2012 to 2014), forty-nine biopsy samples were collected during colonoscopy from the patients with colorectal polyps and ten healthy subjects for normalization. A questionnaire including clinical and demographic data was filled out for all cases. Mucosal mRNA expression of CD86 gene was investigated using Real-Time PCR and Fold Change of gene expression (2-DDCt) method. Results: A total of 49 patients including 27(55.2%) male and 22(44.8%) female with mean age of 53±15.3 were evaluated in this study. Adenoma and hyperplastic polyps were reported in 69.3% and 30.7% patients respectively. More than 90% of adenomatous polyps were spread out to the colon while the others were located in the rectum. Based on Relative Quantitation method, over-expression of CD86 genes in colon polyps were observed (Polyp ≥2-fold normal) and down-expression of CD86 in rectum polyps were noticed in comparison with normal samples (Polyp≤0.5-fold normal). Conclusion: Comparing mRNA expression level of CD86 gene in colon polyps with the profile of mRNA expression of polyps in the rectum indicates the presence of distinct molecular and immunological mRNA expression between polyps of these two sites.

Keywords

• Colorectal Neoplasm
• Colonic Polyp
• Gene Expression
• Antigens CD86