Fructose-1, 6-bisphosphatase 1 inhibits invasion and aerobic glycolysis of colon cancer SW480 cells

Abstract

Objective To determine the expression of fructose-1, 6-bisphosphatase 1 (FBP1) in colon cancer tissues, and investigate its roles and underlying mechanisms in the invasion and aerobic glycolysis of colon cancer cells. Methods The expression of FBP1 was detected by tissue microarray combined with immunohistochemical staining, and then the results were statistically analyzed with clinical pathological parameters. In the colon cancer SW480 cells with vector control, FBP1 overexpression or knock-down, CCK-8 assay was used to evaluate cell proliferation, Transwell chamber test was employed to detect their cell invasion ability, and cell energy metabolism test was applied to measure cell glycolysis. Results The higher expression of FBP1 in 80 cases of colon cancer tissues was significantly lower than that in 80 para-cancerous tissues (21.25% vs 86.25%, P < 0.01). The results of CCK-8 assay showed that FBP1 inhibited the proliferation of SW480 cells (P < 0.01). Transwell assay indicated that FBP1 inhibited the invasion of SW480 cells (P < 0.01). Cell energy metabolism test showed that FBP1 suppressed the level of glycolysis in SW480 cells. Conclusion FBP1 is lowly expressed in colon cancer tissues compared to para-cancerous tissues. FBP1 inhibits migration, invasion and aerobic glycolysis of colon cancer SW480 cells fructose-1 6-bisphosphatase

Keywords

• colon cancer
• invasion
• aerobic glycolysis
• fructose-1 6-bisphosphatase