Preserving the adaptive salt stress response activity of a tissue-specific promoter with modulating activity

Abstract

Background: The Arabidopsis “Redox Responsive Transcription Factor1” (RRTF1) promoter is transiently activated by salt stress in roots over 6 h period, followed by an adaptation phase during which its activity returns to baseline levels, even if the salt stress is prolonged. This enables the short-term production of genes that, while initially advantageous to the plant, will have long-term detrimental effects if expressed at high levels indefinitely. Results: In this paper, we demonstrate that the RRTF1 promoter salt adaption response is a dominant feature of the promoter, that cannot be overwritten by a strong enhancer. While maintaining the transient activation profile of the RRTF1 promoter, linking it to the 35S enhancer results in a significant boost of salt stress induction in roots. Conclusion: The RRTF1 promoter’s enhanced and still adaptable activity could become a useful tool in plant biotechnology.

Keywords

• Salt tolerance
• Transgenic Arabidopsis
• Adaptive promoter
• 35S enhancer