Activation of stably silenced genes by recruitment of a synthetic de-methylating module

Wing Fuk Chan; Hannah D. Coughlan; Yunshun Chen; Christine R. Keenan; Gordon K. Smyth; Andrew C. Perkins; Timothy M. Johanson; Rhys S. Allan

doi:10.1038/s41467-022-33181-4

Nature Communications (Sep 2022)

Activation of stably silenced genes by recruitment of a synthetic de-methylating module

Wing Fuk Chan,
Hannah D. Coughlan,
Yunshun Chen,
Christine R. Keenan,
Gordon K. Smyth,
Andrew C. Perkins,
Timothy M. Johanson,
Rhys S. Allan

Affiliations

Wing Fuk Chan: The Walter and Eliza Hall Institute of Medical Research
Hannah D. Coughlan: The Walter and Eliza Hall Institute of Medical Research
Yunshun Chen: The Walter and Eliza Hall Institute of Medical Research
Christine R. Keenan: The Walter and Eliza Hall Institute of Medical Research
Gordon K. Smyth: The Walter and Eliza Hall Institute of Medical Research
Andrew C. Perkins: Australian Centre for Blood Diseases, Monash University
Timothy M. Johanson: The Walter and Eliza Hall Institute of Medical Research
Rhys S. Allan: The Walter and Eliza Hall Institute of Medical Research

DOI: https://doi.org/10.1038/s41467-022-33181-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 9

Abstract

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Stably silenced genes with methylated CpG at the promoter are refractory to current CRISPR activation systems. Here the authors create a more robust activation system, TETact that recruits DNA-demethylating TET1 with transcriptional activators.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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